Glutathione (GSH/GSSG), HPLC
Glutathion-S-transferases (GST) are a group of enzymes, which are involved in detoxification processes. The GSTs are divided in 5 subgroups: Alpha, Mu, Pi, Teta and microsomal. The classes differ in their physicochemical, immunological, enzymatic and structural properties. Their function in cellular metabolism is the coupling of electrophile compounds, for example most of the carcinogenes, to Glutathion (GSH). The GST Pi is the main group in erythrocytes and in some leucocytes, moreover they are found in most tissues except liver. The GST Pi are dimeric molecules with a molecular weight of 46000 Da and an isoelectric point of about 4,7. High GST Pi levels were found in all neoplastic tissue (fetal, maligne) and in serum of patients with post-operative recidives. Increased serum levels were also found in haematological diseases (haemolytic anaemia and sub-groups of leukemia). Moreover GST Pi concentrations give informations about the multi-drug-resistance-status of tumours.