Test-Category
LC-MS/MS
Pack size
100 Tests
Possible sample matrix and volumes
| Plasma | 50 µl |
| Serum | 50 µl |
Pregnenolone sulfate LC-MS/MS is for the LC-MS/MS based quantification of pregnenolone sulfate in human serum and plasma after precipitation reaction. The assay is an in vitro diagnostic medical device for manual or automatic use by professional laboratory staff in a laboratory environment. The assay serves as an aid in monitoring pregnenolone sulfate-dependent physiological states in endocrine or neurological disorders.
Test principle
A precipitation reaction is used for pregnenolone sulfate enrichment. After chromatographic separation, the analyte is measured via tandem mass spectrometry (MS/MS) in multiple reaction monitoring (MRM) mode.
A stable isotope-labelled version of the analyte is used as internal standard to compensate variances during the sample preparation and matrix effects.