Retinol-binding Protein (RBP/RBP4) (1-point-calibration) ELISA

Article number: K 6120

Test-Category

ELISA

Pack size

96 Tests

Standard concentrations included in the kit

1.1-33 µg/l

Possible sample matrix and volumes

Plasma 20 µl
Serum 20 µl
Urine 100 µl

Incubation periods

  1. 1 hour
  2. 1 hour
  3. 20 minutes

Indications

Regulatory Status

CE marked

Retinol binding Protein (RBP) is small (21kD) transport protein for vitamin A which forms a complex with pre-albumin in blood but looses its affinity for prealbumin once the vitamin has been deliverd to the target cells. The free RBP molecule is rapidly filtered at the glomerulus and catabolized in the renal tubules after resorption by the proximal tubular cells (like other samll molecules e.g. ß-2 Microglobulin). In kidney disease with prevailing tubular changes these proteins are not reabsorbed and appear in the urine.
As actually published by Yang et al. (Nature [2005] 436: 356-362) the plasma form of the RBP, RBP4, seems to play a key role in the development of insulin resistence. The fat cell derived peptide RBP4 which contributes to the transport of vitamin A into the circulatory system for subsequent use also modulates the glucose homeostasis and impairs the insulin sensitivity / insulin resistence. The elevation of serum RBP4 causes systemic insulin resistance, and the reduction of serum RBP4 improves insulin action.

Our RBP ELISA determines the free RBP4 form as well as complex bound RBP4 (RBP4 - prealbumin complex).
This ELISA version (K 6120) is based on a 1-point-calibration.

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